The Lineweaver-Burk plot is a graphical illustration of the Michaelis-Menten equation, which describes the connection between the response price of an enzyme-catalyzed response and the substrate focus. The alpha worth in a Lineweaver-Burk plot is the x-intercept and represents the damaging inverse of the Michaelis fixed (Okaym). The Okaym worth is a measure of the affinity of the enzyme for its substrate, and a decrease Okaym worth signifies a better affinity. Due to this fact, a better alpha worth signifies a decrease Okaym worth and a better affinity of the enzyme for its substrate.
The Lineweaver-Burk plot is a useful gizmo for figuring out the kinetic parameters of an enzyme-catalyzed response. It may be used to find out the Vmax, the utmost response price, and the Okaym, the Michaelis fixed. The Vmax is the utmost velocity of the response, and it’s reached when the enzyme is saturated with substrate. The Okaym is the substrate focus at which the response price is half of the Vmax.
